LAB REPORT OF MICROBIOLOGY 3 Akbar Haqi Academia edu

This preview shows document pages 6 - 7. Sign up to view the full document. This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion, different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify the basic shape and size of the microbe. In this experiment, the microbes E. Coli, Bacillus sp and an unknown microbe from petri dish 6 were categorised as gram negative or gram positive and their shape was identified.

TA LISA Gram Stain Lab Report

These staining techniques are the correct way to identify the shape and size of microbes. The experiment was carried out to use a bright field microscopy, prepare and observe bacterial slides and perform staining methods and explain the mechanism of the bacteria. In order to observe and investigate microbes we need to use microscope and bacterial Staining Techniques. Microscope is the invaluable tool allows the viewing of objects or structures that otherwise would go unnoticed by human naked eyes. In addition, microscope can magnify objects up to 6555 times, revealing microscopic details. It has special techniques and optics thus it can reveal the structure and biochemistry of living cells.

Microscope consists of a combination of several optical lenses. In this experiment, we are using light microscope. Light is conducted through curve lenses in such a way that an object may be viewed larger than its actual size. The light microscopes in this experiment have ocular lenses with magnification of 65X. Moreover, there are also four different objective lenses to choose from 65X, 95X, and 655X. For Bacterial Staining Method, there are two basics techniques.

Gram Stain Lab Report Free Essays StudyMode

One of them is using wet mount method but bacteria are too small and too transparent to be well described using light microscopy and a wet amount. Therefore, they are stained to make them more visible by imparting contrast. Simple stain, with only one layer of cell, is coloured with different colour and methylene blue dye are used for differentiating Bacillus sp. Negative stain is particularly useful for determining cell size and arrangement and it can be used to stain cells that are too delicate to be heat-fixed. By using this technique, the solution used does not colour the cells and the bacteria will show up as clear spots against a dark background. The Gram Staining Method is used as a tool for differentiation of Gram-positive and Gram-negative bacteria, as a first step to determine the identity of a particular bacterial sample.

Gram-positive and Gram-negative organisms are distinguished from each other by differences in their cell walls, including the way the cell takes up and retains stains. The E. Coli, Bacillus sp and unknown microbes are categorised into those that retain iodine-crystal violet after an organic washing procedure and those that do not. Gram Staining is the most consistent when done on bacteria that less than 79 hours, while the older cultures may not retain the primary stain and give inaccurate results. 7. 5 Literature Review This preview shows document pages 6 - 9.

To detect the presence and identify the general type of or sometimes (microbes) in a sample taken from the site of a suspected infection to generally classify bacteria grown in culture so that further identification tests can be performed and appropriate treatment givenWhen a healthcare practitioner suspects that you have a bacterial (or sometimes fungal) infection often whenever a culture is requestedPus, body fluid, sputum, or swab of cells taken from the site of an infection a sample of bacteria or fungi grown and isolated in cultureYou may be able to find your test results on your laboratory's website or patient portal.

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